Publication with datasets Lee et al. 2011, Polymorphism in the couch potato gene clines in eastern Australia but is not associated with ovarian dormancy in Drosophila melanogaster, Molecular Ecology

Keywords
Drosophila melanogaster, cpo, Cline, Diapause and Dormancy

Summary

ABSTRACT: Natural selection can generate parallel latitudinal clines in traits and gene frequencies across continents, but these have rarely been linked. An amino acid (isoleucine to lysine, or I462K) polymorphism of the couch potato (cpo) gene in Drosophila melanogaster is thought to control female reproductive diapause cline in North America (Schmidt et al. 2008, Proc Natl Acad Sci USA, 105, 16207-16211). Here, we show that under standard diapause-inducing conditions (12 °C and short photoperiod) (Saunders et al. 1989, Proc Natl Acad Sci USA, 86, 3748-3752), egg maturation in Australian flies is delayed, but not arrested at previtellogenic stages. At 12 °C, the phenotypic distribution in egg development was bimodal at stages 8 and 14 and showed a strong nonlinear pattern on the east coast of Australia, with incidence of egg maturation delay (ovarian dormancy) increasing both toward tropical and temperate climates. Furthermore, we found no evidence for an association between the cpo I462K polymorphism and ovarian dormancy at either 12 or 10 °C (when egg maturation was often delayed at stage 7). Owing to strong linkage disequilibrium, the latitudinal cline in cpo allele frequencies was no longer evident once variation in the In(3R)P inversion polymorphism was taken into account. Our results suggest that the standard diapause-inducing conditions (12 °C and short photoperiod) were not sufficient to cause the typical previtellogenic developmental arrest in Australian flies and that the cpo I462K polymorphism does not explain the observed delay in egg development. In conclusion, ovarian dormancy does not show a simple latitudinal cline, and the lack of cpo-dormancy association suggests a different genetic basis to reproductive dormancy in North America and Australia.

Datasets

  • 65 Lee et al. 2011, Egg stage 2008

    This data file contains maximun egg stage reached in ovaries of female Drosophila melanogaster after being held at diapause inducing conditions (12˚C; 10L:14D) for 28 days. The females originated from 17 populations collected in 2008 (Fig. 2a&b, 3a&b).

  • 66 Lee et al. 2011, Egg stage 2009

    This data file contains maximun egg stage reached in ovaries of female D. melanogaster after being held at diapause inducing conditions (12˚C or 10˚C; 10L:14D) for 28 days. The females originated from Melbourne and Innisfail and were collected in 2009 (Fig. 2a&b, 3a&b).

  • 67 Lee et al. 2011, Egg stage 2010

    This data file contains maximun egg stage reached in ovaries of female D. melanogaster after being held at diapause inducing conditions (12˚C or 10˚C; 10L:14D) for 28 days. The females originated from Melbourne and Innisfail and were collected in 2010 (Fig. 2a&b, 3a&b).

  • 68 Lee et al. 2011, Association analysis

    This data file contains maximun egg stage reached in ovaries of female D. melanogaster after being held at diapause inducing conditions (12˚C or 10˚C; 10L:14D) for 28 days and genotypes of the couch potato locus (I = cpo-462I homozygote; K = cpo-462K homozygote; IK = cpo-462I/K heterozygote). Maximum egg stage and cpo genotype were used for the genotype-phenotype association analysis. The females originated from Melbourne and were collected in 2009.

  • 69 Lee et al. 2011, Couch potato locus

    This data file contains numbers of genotypes of the couch potato locus (I = cpo-462I homozygote; K = cpo-462K homozygote; IK = cpo-462I/K heterozygote) and frequencies of the K (cpo-462K) genotype of 17 D. melanogaster populations collected in 2005. Genotypes and frequencies are given within the inverted and standard arrangement of the In(3R)P inversion and overall (Fig. 4).

  • 70 Lee et al. 2011, Couch potato expression

    This data set contains expression levels of the couch potato (cpo) locus relative to the housekeeping gene RpL11 in 4-day old females of 15 D. melanogaster populations collected in 2008 (Fig. 5).

Publication Citations

  • Lee, Siu F., Sgrò, Carla M., Shirriffs, Jennifer, Wee, Choon W., Rako, Lea, van Heerwaarden, Belinda, and Hoffmann, Ary A., 'Polymorphism in the couch potato gene clines in eastern Australia but is not associated with ovarian dormancy in Drosophila melanogaster', Molecular Ecology, vol. 20, no. 14, 2011, pp. 2973-2984. http://dx.doi.org/10.1111/j.1365-294X.2011.05155.x. Details
  • Lee, Siu F., Sgrò, Carla M., Shirriffs, Jennifer, Wee, Choon W., Rako, Lea, van Heerwaarden, Belinda, and Hoffmann, Ary A., Data from: Polymorphism in the couch potato gene clines in eastern Australia but is not associated with ovarian dormancy in Drosophila melanogaster, Dryad Digital Repository, 2011. http://dx.doi.org/10.5061/dryad.k175g. Details